MAPTrix HyGelTM - The first user-defined three-dimensional (3D) matrix based on biochemical & physical cues
Currently existing products, such as extracted and reconstituted basement membrane, have associated with them a diverse array of unknown cues which can be highly disadvantageous, particularly when trying to isolate the effects of specific factors. In addition, these products generally have a variable composition as well as variable mechanical properties. Furthermore, lot-to-lot inconsistency of reconstituted basement membrane results in significant limitations regarding their end-use.
Kollodis BioSciences addresses these limitations by providing a user-defined and in situ formable hydrogel system called MAPTrix HyGelTM. Our MAPTrix HyGelTM line of products readily generate three-dimensional (3D) extracellular matrix environments for 3D cell culture applications.
MAPTrix HyGelTM is a recombinant mussel adhesive protein based, semi-synthetic 3D extracellular matrix that is tailored to mimic specific ECM (extracellular) properties. The MAPTrix HyGelTM hydrogel products are composed of two components: MAPTrixTM ECM, a mussel adhesive protein based extracellular matrix (ECM) mimetic; and, MAPTrixTM Link, a multi-arm polyethylene glycol derivative. Use of MAPTrix HyGelTM generates a well-controlled and reproducible cellular environment for your 3D cell culture and related application. Download MAPTrix HyGel™ Product Overview (Adobe Acrobat PDF File)
Highlighted Features of MAPTrix HyGelTM
MAPTrix HyGelTM is an innovative and versatile 3D cell culture tool that addresses the unmet needs of a chemically and physically defined matrix for cell culture applications and includes the following notable features:
in situ creation of a fully-defined 3D extracellular matrix
- Free of animal derived components
- Readily able to engineer the elasticity and/or pore size of the hydrogel by adjusting the concentration of MAPTrixTM ECM or MAPTrixTM Linker
User-friendly, ready-to-use formulation for the end-user
- All components of MAPTrix HyGel™ are relatively stable and can be stored in a refrigerator, no deep-freezing is required
- Simply mixing each component can form a hydrogel within two hours, under physiological conditions
- All components are soluble in most buffer solutions
- MAPTrix HyGel™ is compatible with existing cell culture protocols
High lot-to-lot consistency and reproducibility
- High batch-to-batch reproducibility between samples and vials
- QC & QA testing in accordance with USP Guidelines
Engineering A Well Defined 3D ECM with MAPTrix HyGelTM
The inherent flexibility of MAPTrix HyGelTM for engineering biochemical and physical characteristics in a modular way allows the end-user to adapt MAPTrix HyGelTM for a variety of cell types and biological applications.
The MAPTrix HyGelTM matrix can be modularly engineered for designed biochemical characteristics by use of either a single MAPTrixTM ECM product or a combination of MAPTrixTM ECM products. Kollodis currently provides many different MAPTrixTM ECM products in its expanding MAPTrixTM ECM line of products for engineering the extracellular matrix, as indicated in the following table.
Highlighted Features of MAPTrix HyGelTM
in situ creation of a fully-defined 3D extracellular matrix
